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dc.contributor.authorAli Hussain, Reshak, Prof. Dr.
dc.contributor.authorMukhzeer, Mohamad Shahimin, Dr.
dc.contributor.authorFhataheya, Buang
dc.date.accessioned2014-06-05T06:47:11Z
dc.date.available2014-06-05T06:47:11Z
dc.date.issued2013-11
dc.identifier.citationProgress in Biophysics and Molecular Biology, vol. 113(2), 2013, pages 295-298en_US
dc.identifier.issn0079-6107
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S007961071300093X
dc.identifier.urihttp://dspace.unimap.edu.my:80/dspace/handle/123456789/35114
dc.descriptionLink to publisher's homepage at http://www.elsevier.com/en_US
dc.description.abstractMammalian adipose tissue derived stem cells (AT-SC) have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1h and 2h under 37°C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1h under 37°C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2h under 37°C in CO2 incubator.en_US
dc.language.isoenen_US
dc.publisherElsevier Ltd.en_US
dc.subjectAbdominal subcutaneous adipose tissueen_US
dc.subjectBovineen_US
dc.subjectHumanen_US
dc.subjectStem cell isolationen_US
dc.titleComparative study on human and bovine AT-SC isolation methodsen_US
dc.typeArticleen_US
dc.contributor.urlmaalidph@yahoo.co.uken_US
dc.contributor.urlmukhzeer@unimap.edu.my en_US
dc.contributor.urlfhataheya_buang@yahoo.comen_US


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