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dc.contributor.authorMd. Eaqub, Ali, Dr.
dc.contributor.authorUda, Hashim, Prof. Dr.
dc.contributor.authorShuhaimi, Mustafa
dc.contributor.authorYaakob, Che Man
dc.contributor.authorTijjani Adam, Shuwa
dc.contributor.authorQazi Muhammad, Humayun
dc.date.accessioned2014-05-29T07:27:55Z
dc.date.available2014-05-29T07:27:55Z
dc.date.issued2014
dc.identifier.citationJournal of Experimental Nanoscience, vol.9 (2), 2014, pages 152-160en_US
dc.identifier.issn1745-8080 (print)
dc.identifier.issn1745-8099 (online)
dc.identifier.urihttp://dspace.unimap.edu.my:80/dspace/handle/123456789/34870
dc.descriptionLink to publisher's homepage at http://www.tandfonline.comen_US
dc.description.abstractA 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene was integrated to 3-nm diameter citrate–tannate-coated gold nanoparticles to fabricate a species-specific nanobiosensor. The biosensor was applied to authenticate pork adulteration in meatball formulation, which is a favourite food in many Asian and European countries. The sensor was found to be sensitive enough to detect 1% pork in raw and cooked meatballs, prepared from the previously mixed pork and beef in specific ratios (% w/w). The hybridisation kinetics of the hybrid biosensor was studied with synthetic targets from moderate to extreme target concentrations and a hyperbolic relationship was found. However, linearity was observed with probe/target ratios 4:1 to 1:2. This part of the curve quantified target DNA in ready-to-eat mixed meatball preparations with more than 90% accuracy. The biosensor probe was hybridised with a target DNA that was several-fold shorter than a typical PCR-template. This offered the detection and quantitation of potential targets in highly processed meat products or extensively degraded samples where PCR-based identification technique might not work due to the fragmentation of comparatively longer DNA. We believe that the assay can be used as an alternative to qPCR for determining shorter size DNA sequences in degraded samples to address a range of biological problems, such as food analysis, bio-diagnostics, environmental monitoring, genetic screening and forensic investigations.en_US
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.subjectHybrid nanobiosensoren_US
dc.subjectHybridisation kineticsen_US
dc.subjectHyperbolic relationshipen_US
dc.subjectEmulsified meaten_US
dc.subjectTemplate DNA stabilityen_US
dc.titleNanobiosensor for the detection and quantification of pork adulteration in meatball formulationen_US
dc.typeArticleen_US
dc.identifier.urlhttp://www.tandfonline.com/doi/full/10.1080/.U34cUHY0qho#preview
dc.contributor.urleaqubali@gmail.comen_US


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