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dc.contributor.authorNoorhaslina, Hashim
dc.contributor.authorAbdul Razak, Shaari
dc.contributor.authorAwang Soh, Mamat
dc.contributor.authorSyarhabil, Ahmad
dc.date.accessioned2020-12-23T09:22:59Z
dc.date.available2020-12-23T09:22:59Z
dc.date.issued2016
dc.identifier.citationMATEC Web Conferences, vol.78, 2016, 7 pagesen_US
dc.identifier.issn2261-236X (online)
dc.identifier.urihttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/69149
dc.descriptionLink to publisher's homepage at https://www.matec-conferences.org/en_US
dc.description.abstractBioactive compounds of Orthosiphon stamineus have been known to have the beneficial effects on health. Efficient extraction of these beneficial compounds is very important. The study was conducted to determine the efficiency of methanol as an extraction solvent for bioactive compound extraction of O. stamineus raw materials. Leaf sample was extracted in 25, 50 and 100% methanol and distilled water for 2, 4 and 8 hours at 40°C. The free radical scavenging activity (FRSA) method was used to determine antioxidant capacity and Follin-Ciocalteu method was used to determine samples total phenolic compounds. The quantification of bioactive constituents by using the high performance liquid chromatography (HPLC) and the standard markers was used for this analysis such as rosmarinic acid (RA), sinensetin (SEN) and 3-hydroxy-5,6,7,4-tetramethoxyflavone (TMF). The current research shows that the yield of RA concentration was high in 50% methanol extracts at 2, 4 and 8 h of extraction. For the FRSA result showed that methanol at 50% and 100% was the most effective concentration levels inhibition between 45-55% at 8 hours’ time extraction. For the total phenolic compound, the 50% methanol concentration in O. stamineus leaf had the highest value compared with 0%, 25% and 100% methanol concentration. But, it was observed that time extraction did not influence the TPC values for different concentrations of methanolic extracts. These indicated that time extraction had affected the total capacities of antioxidant and 50% methanolic extracts was the best concentration for indicated the TPC in the O. Storminess leaf.en_US
dc.language.isoenen_US
dc.publisherEDP Sciencesen_US
dc.relation.ispartofseries2nd International Conference on Green Design and Manufacture 2016 (IConGDM 2016);
dc.subjectOrthosiphon stamineusen_US
dc.subjectBioactive compoundsen_US
dc.titleEffect of differences Methanol concentration and extraction time on the antioxidant capacity, phenolics content and bioactive constituents of Orthosiphon Stamineus extractsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1051/matecconf/20167801004
dc.contributor.urlleein16@gmail.comen_US


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