Optimization of extraction conditions for antioxidant activity of crude bioactive phenolic from phanerochaete chrysosporium by liquid-state fermentation

Abstract

Phanerochaete chrysosporium known as white rot fungus has the ability to degrade the abundant aromatic polymer lignin. In this study, the extractions for antioxidant activity of Phanerochaete chrysosporium biomass extract were determined by using Central Composite Experimental Design. The extraction conditions studied were solid-to-solvent ratio, extraction pH and extraction time, while agitation speed, temperature and solvent concentration was set at the minimum value. All three parameters used give significant value which are then can be proceed for the optimization process. Folin-Ciocalteu reagents were used to determined the total phenolic content while 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging assay was used to analyze the antioxidant activity. From the result, there were no correlation found between total phenolic compounds and antioxidant activity. Phanerochaete chrysosporium biomass extract demonstrated as most potential free radical scavenging activity showing 82.80 % compares with ascorbic acid (Vitamin C) that showed 54.25 %. The optimization process meets the conditions at extraction pH 3.28, solid-tosolvent ratio 15.89 and extraction time 167.91, as the result showed the antioxidant activity is 81.98 % just slightly closed to the predicted value 82.27 %.