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dc.contributor.authorThikra S., Dhahi
dc.contributor.authorMd Eaqub, Ali, Dr.
dc.contributor.authorUda, Hashim, Prof. Dr.
dc.contributor.authorNor Azah, Yusof
dc.contributor.authorIhsan M., Ali
dc.contributor.authorM. J., Ashoor
dc.contributor.authorA., Al-Roumy
dc.contributor.authorH.A, Hadi
dc.date.accessioned2014-03-21T07:20:41Z
dc.date.available2014-03-21T07:20:41Z
dc.date.issued2013
dc.identifier.citationCurrent Nanoscience, vol. 9(2), 2013, pages 283-287en_US
dc.identifier.issn1573-4137
dc.identifier.urihttp://www.eurekaselect.com/109029/article
dc.identifier.urihttp://dspace.unimap.edu.my:80/dspace/handle/123456789/32971
dc.descriptionLink to publisher's homepage at http://www.benthamscience.com/en_US
dc.description.abstractThis report described a polysilicon nanogap biosensor for the detection of target DNA hybridization which is a key step in biodiagnostics, gene expression profiling, environmental monitoring and forensic investigation. The detection was performed with a low cost dielectric analyzer which measured the changes in capacitance, conductance and permittivity of the nanogap electrodes upon target DNA hybridization. A conventional lithography coupled with thermal oxidation-based size reduction technique was used to fabricate the polysilicon nanogap electrodes. A layer of self-assembled amine functionalities coupled with non-covalently adsorbed gold nanoparticles was added onto the nanogap surface to create a binding chemistry for the thiol-modified probe DNA and to enhance the detection signal. The hybridization detection discrimination among the complementary, noncomplementary and single mismatch targets was reflected through the differences in capacitance, conductance and permittivity profiles of the biosensor. The detection limit of the polysilicon nanogap biosensor was 5 nmol/L of target DNA.en_US
dc.language.isoenen_US
dc.publisherBentham Science Publishers.en_US
dc.subjectCapacitanceen_US
dc.subjectConductivityen_US
dc.subjectDNA detection discriminationen_US
dc.subjectNanogap biosensoren_US
dc.subjectPermittivityen_US
dc.titleDNA hybridization detection using 5-nm polysilicon nanogap structureen_US
dc.typeArticleen_US
dc.contributor.urlsthikra@yahoo.comen_US
dc.contributor.urleaqubali@um.edu.myen_US
dc.contributor.urluda@unimap.edu.myen_US


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