Study of Saccharomyces Cerevisiae Concentration Dependence on the Bioethanol Production Using Rotary Evaporator

Abstract

The use of fossil fuel energy has a negative impact on the environment by causing energy pollution and also triggers the global warming problem. Therefore, an alternative of fuel energy sources is highly required. Currently, the alternative fuel energy such as bioethanol has gained a lot of attention. Bioethanol is ethanol resulted from the fermentation of raw materials such as vegetable. The purpose of this work is to investigate the relationship between fermentation time, ratio of organic vegetable waste to water (R) and Saccharomyces Cerevisiae concentration on the quantity of bioethanol production. The vegetable waste collected from one of the markets in Medan was used as the main material in this experiment. The bioethanol produced from samples with various experimental conditions were analyzed by Gas Chromatography (GCMS). The results indicate that the optimum ratio of organic vegetable waste and water to produce the highest bioethanol concentration is 3:1. The samples containing 6% Saccharomyces Cerevisiae with 6 - 10 days fermentation produced ca. 11 - 30% bioethanol. On the other hand, those with 10% Saccharomyces Cerevisiae with 6 - 10 days fermentation produced ca. 13 - 33% bioethanol.