Optimization study of factors affecting Cellulase production by Anoxybacillus sp. Strain UniMAP KB-01 from tropical mangrove soil

Abstract

Cellulase is an important enzyme that has various industrial applications, such as agriculture, bioconversion, detergents, fermentation, food, pulp and paper, textile, and others. The abundance of cellulose as the most dominating agricultural waste has grabbed the attention of researchers worldwide. This research was aimed to utilize the bacteria isolated from the tropical mangrove soil, which has abundant cellulose, to undergo cellulolytic reaction, breaking down cellulose into glucose. Such bacteria is named Anoxybacillus sp. strain UniMAP KB-01. The optimization of this bacteria enables to maximize the production of cellulase by cultivating it in specific conditions. The significant factors that were considered in the experimental design include temperature, pH, and concentration of CaCl2. These significant factors were subjected to optimization studies using Response Surface Methodology (RSM) which involve Central Composite Design in Design-Expert (DOE) software. Maximum enzyme activity was able to be obtained at a temperature of 55°C, pH of 6.17 and concentration of CaCl2 of 0.14 g/L, which was 40.4 mU/mL.