| dc.description.abstract | Spider silks have many excellent mechanical properties especially in terms of tensile strength and elasticity in comparison with other silks sources. Due to these exceptional properties, many potential applications arise as spider silk can be served as an advantageous biomaterial in real industry. Though the usefulness of spider silks are acknowledge by researches, only certain species such as Nephila and Araneus spiders had been studied while the rest of the species remain unknown and unexplored. Primarily, the present study aims to optimize the extraction process of dragline silk protein from Crossopriza lyoni webs. Initially, degumming process of C. lyoni webs was conducted using 0.02M sodium carbonate for 30 minutes boiling. Then, the screening of the best extraction solvent between Ajisawa’s and sodium dodecyl sulphate (SDS) solution under constant condition was continued and resulted in SDS solution as the best extraction solvent. Subsequently, the screening study of significant parameters such as SDS concentration, temperature and pH affecting the spidroin extraction process was continued using Full Factorial Design with the resulted best extraction solvent. 1503.75 μg/ml of protein was extracted under 10% SDS concentration, 80ºC and pH 5 treatment. Then, optimization of spidroin extraction was carried out by Response Surface Methodology (RSM) via Box Behnken Design (BBD) that resulted in the highest protein concentration of 4333 μg/ml under 15% SDS concentration, 90ºC and pH 5 treatment. SDS-PAGE was conducted with the use of the highest protein concentration sample as a spidroin validation step and resulted in bands with the molecular weights of more than 200 kDa. Since Malaysia have vast population of many spider species, this present research can be considered as valuable and may become the benchmark for future exploration of spider silks in the innovation of useful products and applications. | en_US |
