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    Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)

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    Date
    2008-09-22
    Author
    A.L., Ahmad
    C.J.C., Derek
    Mohamed Zulkali, Mohamed Daud
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    Abstract
    The work attempts to study and optimize aqueous two-phase system (ATPS) made of polyethylene glycol (PEG)/sodium sulfate and PEG/potassium phosphate. Five factors (PEG's molecular weight, PEG's concentration, pH, concentration of sodium chloride and phase-forming salt) affecting the thaumatin partitioning were studied. A two-level fractional factorial was initially carried out. The statistical analysis showed that for both the systems, the phase-forming salt concentration and the concentration of sodium chloride significantly affects the K value for thaumatin partitioning. However, the performance of PEG/sodium sulfate system was generally better than the PEG/potassium phosphate system. Hence a detail study was carried out on the PEG/sodium sulfate system using central composite rotatable design (CCRD) in response surface methodology (RSM). It was observed that high salt concentration increased the K value by sevenfold and the PEG molecular weight was not a significant factor. The optimal condition gave a K value for thaumatin partitioning of 24.15 with a yield of 96.02%.
    URI
    http://www.sciencedirect.com/science/journal/13835866
    http://dspace.unimap.edu.my/123456789/6673
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