| dc.contributor.author | Che Wan Sharifah Robiah, Mohamad | |
| dc.contributor.author | Uyub, Abd Manaf | |
| dc.contributor.author | Yahya, Mat Arip, Dr. | |
| dc.date.accessioned | 2013-10-21T03:21:16Z | |
| dc.date.available | 2013-10-21T03:21:16Z | |
| dc.date.issued | 2012-06-18 | |
| dc.identifier.citation | p. 907 - 910 | en_US |
| dc.identifier.isbn | 978-967-5760-11-2 | |
| dc.identifier.uri | http://dspace.unimap.edu.my/123456789/28988 | |
| dc.description | The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012) jointly organized by Universiti Malaysia Perlis and Athlone Institute of Technology in collaboration with The Ministry of Higher Education (MOHE) Malaysia, Education Malaysia and Malaysia Postgraduates Student Association Ireland (MyPSI), 18th - 19th June 2012 at Putra World Trade Center (PWTC), Kuala Lumpur, Malaysia. | en_US |
| dc.description.abstract | Helicobacter pylori is a gram-negative bacterium that colonizes the gastric mucosal in human stomach. It causes gastric ulcers and cancer by mechanisms that are not well understood. Helicobacter pylori produces enzyme that damage gastric epithelial cells, such as urease (constitute up to 10% of total bacterial protein) that neutralize the acidic medium permitting its survival in the human’s stomach. Urease (urea amidohydrolase; EC 3.5.1.5) is a nickel-dependent enzyme that catalyzes the hydrolysis of urea to form ammonia and carbon dioxide. The genes for urease enzymes are organized in a gene cluster or operon. In this preliminary work, the urease operon was amplified using polymerase chain reaction (PCR) and cloned into pET 32Ek/LIC (Invitrogen, USA) system. The aim of this study is to obtain a functional replicon of urease operon that capable of expressing the urease enzymes and introduce the replicon in animal model for pathogenesis, as well as, pathophysiological studies of Helicobacter pylori. | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Malaysia Perlis (UniMAP) | en_US |
| dc.relation.ispartofseries | Proceedings of the The 2nd International Malaysia-Ireland Joint Symposium on Engineering, Science and Business 2012 (IMiEJS2012); | |
| dc.subject | Helicobacter pylori | en_US |
| dc.subject | Urease operon | en_US |
| dc.subject | pET 32 Ek/LIC | en_US |
| dc.title | Cloning of urease operon from helicobacter pylori J99 | en_US |
| dc.type | Working Paper | en_US |
| dc.contributor.url | ymarip@usm.my | en_US |
