| dc.description.abstract | The ethanolic extractions from the parts (seeds and leaves) of Cerbera odollam were
used in order to verify the antimicrobial activity and cytotoxicity by using different
concentration of dilution prepared. The antimicrobial test was done to evaluate
percentage of inhibition zone against Fusarium sp. colony growth on media with
different concentration of extracts dilution. The concentrations prepared were in 500
ppm, 1625 ppm, 2750 ppm, 3875 ppm, and 5000 ppm after all the concentration being
selected by using One Factor solution under Response Surface Methodology on Design
of Expert software. The highest concentration of dilution of seeds and leaves at 5000
ppm were show highest percentage of inhibition zone, 86.67% (seeds) and 66.7%
(leaves). For brine shrimp bioassay, the LC50 have been determining by appliying the
ethanolic extraction with different concentration on brine shrimp (Artemia salina
larvae). The concentrations used also have been determined by using One Factor
solution under Response Surface Methodology on Design of Expert software until
provided 10 ppm, 167.5 ppm, 325 ppm, 482.5 ppm and 640 ppm. The seeds and leaves
of Cerbera odollam have killed 50 % of brine shrimp population tested at 190.54 mg/ml
and 117.49 mg/ml, respectively which are indicated as LC50 values of Class IV of
toxicity category, that are environmentally safe but the concentration should be used
with caution. | en_US |
