Optimization of process condition for bioprotein production in shake flask study

Abstract

Bioprotein is a protein that is cultivated from many types of microorganisms through fermentation process, either solid state fermentation or liquid state fermentation. The microorganism used was white rot fungus, Phanerochaete chrysosporium. The aim of this research is to optimize the process condition in order to produce high biomass of bioprotein in shake flask study. In addition, it is desired to study the effect of the interaction between the parameters, agitation speed, inoculum size and the initial pH. The research was done by using Central Composite Design (CCD) under Response Surface Methodology (RSM). Based on the screening of fermentation period, six days of incubation period was considered as the optimum time for submerged fermentation. In the optimization process, the design contains a total of 17 experimental runs at three level systems. From the analysis of variance (ANOVA) using quadratic polynomial equation, maximum bioprotein production and total protein content of obtained was 4.698g/L and 5.3345 mg/ml respectively by using 150 rpm, with 5.5% inoculum size at pH 5. The validation of model was done and showed that the model are valid as 4.469g/L bioprotein production and 4.5735 mg/mL of total protein respectively are obtained from the validation experiment.