Optimisation of solvent extraction of lipid from earthworm powder
Abstract
The main objective of this study was to see the effect of amount of hexane and the length of
extraction time for the extraction of lipid from earthworm powder. Lipids were isolated in
the Soxhlet apparatus. Factorial design of experiment using Stat-Ease 8.0.1 software was
studied as a way to study the effect of the lipid extraction from the earthworm powder. In
order to find the significant variables affecting the lipid extraction from earthworm powder,
two level factorial designs were introduced to screen out the variable key. The impact of the
two factors such as length of extraction time and the amount of solvent (hexane) was
studied on the yield of the lipid extracted. The amount of solvents examined were 150ml,
175 ml and 200 ml and the extraction time examined were 7 hours, 8 hours and 9 hours.
From the result obtained, the maximum weight of lipid was extracted from the earthworm
powder was 16.018 % (w/w) representing 0.16 per gram of lipid at time 9 hours and 200 ml
of hexane. But, analysis of variance (ANOVA) results using Stat-Ease 8.0.1 software
indicate there was no significant different between the parameter that have been chosen.