Analysis of electrical DNA biosensor for human Papillomavirus (HPV) strain 18 detection in synthesis and saliva samples

Abstract

This project is to focus on the study of the electrical DNA biosensor for the detection of HPV strain 18. In this research, electrical DNA biosensor is expected to detect HPV strain 18 more efficiently by using electrical characterization. In this project, device inspection will be conducted first to make sure the functional of the gold interdigitated electrode (IDE) by using Scanning Electron Microscope (SEM). 3-Aminopropyl Triethoxysilane (APTES) solution was used for the process of surface modification to form the amine group on the surface of the device to facilitate the attachment of DNA probe. In this project, synthetic DNA sample and DNA from saliva of several Bios systems Engineering students were used as the target DNA. The current-voltage (I-V) electrical characterization was conducted to detect the presence of HPV strain 18 in both DNA samples. As the results, perfect alignment between the electrode on the IDE were detected under SEM. Surface modification of the biosensor successfully conducted which is the covalent bond between APTES and DNA probe increase the electrical. Synthetic DNA shows the presence of HPV strain 18 while there were no HPV strain 18 detected in the DNA from saliva samples.