Development of nanogap automated permittivity measurement system for DNA hybridization detection kit

Abstract

The Nanogap Automated Permittivity Measurement System (APMS) are fabricated as low cost, portable and label free DNA hybridization detection kit. The nanogap capacitor can react as a dna sensor. The difference in dielectric properties of singlestranded DNA (SSDNA) and double-stranded DNA (DSDNA) are allowing the use of permittivity measurement for DNA hybridization detection. In this project, an automated measurement system is introduced as an interface for nanogap capacitor. Sine wave frequency from 1Hz until 100kHz will be applied to the nanogap capacitor and it voltage wave and current wave are measured and analyzed. Three measurements will be made, capacitance measurement of nanogap with no sample, permittivity measurement of after immobilization and after hybridization. If the measurement show a considerably difference in permittivity value, the DNA is consider hybridize. Otherwise is it considered mismatch. In this project, an Artificial Neural Network (ANN) will also be introduced for decision making of the result based on the permittivity measurement. This will allow the user to get the result without comparing the output.