Effect of different composition of sugarcane agro-waste media on Mycelium growth

Abstract

Sugarcane bagasse is the fibrous matter that left after sugarcane has been crushed to extract the juices in it during the process of sugar production. The abundant amount of sugarcane waste lead the farmer to burn the waste as it is the easiest way to decompost the sugarcena waste. However, burning would encourage pollution and global warming. Instead of burning, turn the these agricultural waste into substrates to cultivate mushroom is a better way of waste disposal management. This project aims to investigate the best level composition of sugarcane bagasse that could give optimum result for mycelium growth and the effect to the lignin and cellulose content. In this research, the mycelium growth was determined by measuring the length of mycelium. The parameters which are different level composition of substrate were carried out to show the effects of the different composition of rice bran and sugarcane bagasse substrates to the mushroom growth. Different responses test such as sulphuric acid in order to get lignin and ethanol to get cellulose have been conducted. In this project, it showed that the sugarcane with 40 to 50 % of rice bran had the highest mycelium growth and less growing day due to high lignocellulosic content in sugarcane bagasse and rice bran. The highest mycelium is 9.5 cm on day 28. The highest mycelium growth rate is 0.3 cm/30 day in T3. T3 (50:50 sugarcane bagasse and rice bran respectively) also give the lowest day to induce the mycelium. The lignin and cellulose content were increasing from day 0 to the day after spawn running period.