Study of the effect of cold atmospheric plasma on the growth of MCF-7 cell as alternative cancer treatment

Abstract

Cold atmospheric plasma (CAP) is gaining importance due to its functional ability to accelerate and promote apoptosis of tumour cells. Due to this capability, CAP has arise as the potential alternative cancer therapy as recent therapy such chemotherapy has some side effects to the cancer patient after the treatment with the cancer case kept rising from days by days. CAP also has got the researcher’s interest due to its selectivity toward cancer cell compared to normal cell. Primarily, the study aims to investigate the killing effect of cold atmospheric plasma (CAP) on growth of breast cancer cell as alternative cancer therapy. Initially, the breast cancer cell , MCF-7 was cultured in DMEM consists of 10% Fetal Bovine Serum and 1% Penicilin/Streptomycin for nine days to develop the growth curve. Subsequently, the screening study of significant parameters such as time of CAP exposure, the flow rate of helium and distance of CAP exposure was continued using Full Factorial Design with the resulted best condition for maximal cell dead. 58650 cells/ml of dead cell concentration was recorded from treatment for 120 s , 0.5 sl/min of helium and kept distance at 1 cm. Then, the best condition was selected to treat the normal breast cell, MCF-10a and make comparison on the dead cell concentration after the treatment. The result shows that longer time, less helium and shorter distance has recorded the maximal cell dead and CAP was more sensitive towards MCF-7 compared to MCF-10a. This present study can considered as valuable and may become the benchmark for future exploration of capability of CAP in order to be other options for cancer therapy instead of treat with chemotherapy which has some side effects.