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dc.contributor.authorTan, Chee Kang
dc.date.accessioned2015-01-11T08:30:20Z
dc.date.available2015-01-11T08:30:20Z
dc.date.issued2013-06
dc.identifier.urihttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/38465
dc.descriptionAccess is limited to UniMAP community.en_US
dc.description.abstractA hybrid sol-gel carbon nanotubes composite has been developed and used for surface covalent bonding of enzyme for biosensing application. The biosensor was developed by covalent immobilization of glucose oxidase enzyme on sol gel modified electrode with ferrocene via carbodiimide reaction. The presence of amine groups provided by APTMOS in sol gel controlled the covalent bonding sites for enzyme. The experiment conditions such as amount of enzyme and pH in working buffer was studied by using Response Surface Methodology (RSM) through Central Composite Design (CCD) of Design Expert software. The biosensor showed maximum current response at 20 U/mL of immobilized glucose oxidase at the optimum pH value of working buffer of pH 7. The calibration curve of GOx biosensor was linear in the range of 0.1 mM to 3 mM of glucose concentration with a linear equation and correlation coefficient of 0.9873. The performance of GOx biosensor was evaluated based on selectivity toward the interferences and stability, the GOx biosensor exhibited high selectivity behavior and good stability.en_US
dc.language.isoenen_US
dc.publisherUniversiti Malaysia Perlis (UniMAP)en_US
dc.subjectBiosensoren_US
dc.subjectGlucose oxidase (GOX) enzymeen_US
dc.subjectNanotubesen_US
dc.subjectCarbon paste electrodeen_US
dc.titleImmobilization of glucose oxidase enzyme onto organic-inorganic sol-gels matrix on carbon paste electrode biosensoren_US
dc.typeLearning Objecten_US
dc.contributor.advisorDr. A.K.M Shafiqul Islamen_US
dc.publisher.departmentSchool of Bioprocess Engineeringen_US


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