Optimization of media composition for bioprotein production from empty fruit bunch by aspergillus terreus UniMAP AA-1
Abstract
Two steps optimization strategy based on stastisical experimental design was carried
out to enhance bioprotein production by solid state fermentation utilizing empty fruit
bunch (EFB) as substrate. Plackett-Burman design was employed first where 7
variables were studied for their influence in bioprotein production. NH4NO3,
CuSO4.5H2O and MgSO4.7H2O were found to be most significant variables for
improving bioprotein production. Central Composite Design (CCD) was employed to
maximize the production of bioprotein. A mathematical model was developed to show
the effects of each media component and their combinatorial interaction on bioprotein
production. The optimal medium composition for maximum bioprotein production was
are 0.70 % (v/w) of inorganic nitrogen source (NH4NO3), 0.06 % (v/w) of magnesium
source (MgSO4.7H2O) and 0.23 % (v/w) of copper source (CuSO4.5H2O). Validation
fermentation was carried out to validate the model. From the model validation process,
predicted amount of protein concentration produced was 0.606598 mg/mL which is
slightly higher than the experimental run in CCD which was 0.604 mg/mL.