| dc.description.abstract | The methanolic extractions from the parts seeds and leaves of Cerbera odollam were
used in order to verify the antibacterial activity from different concentration of dilution
prepared. Cerbera odollam leaves and seeds were collected nearby the School of
Bipoprocess Engineering in Jejawi. The antimicrobial test was done to evaluate
percentage of inhibition zone against e.coli and Bacillus cereus growth on media with
different concentration of extracts dilution and different incubation time. The
concentrations prepared were in 600 ppm, 800 ppm and 1000 ppm, while for incubation
were from 48 hours, 72 hours and 96 hours. From screening process, seeds extraction
was chosen. The optimizing condition was done for both bacteria by using Response
Surface Methodology in Design of Expert. The results for experimental of optimum
concentration and incubation time for Bacillus cereus was at 800 ppm, at 72 hours with
88% inhibition. Whereas, optimum concentration and incubation time for E. coli was at
800 ppm, at 72 hours with 78 %. From RSM analysis, the optimum process condition
of seeds extract, on Bacillus cereus with concentration of 660 ppm, and incubation time
at 88.80 hours. Then, on E.coli: with concentration at 760 ppm and incubation time at
78.80 hours. | en_US |
