Solid State Bioengineering Production of Schizopyllan by Schizophyllum commune from Rice Straw

Abstract

Polysaccharides seemed to be the most potent mushroom derived substance with antitumor properties. Schizophyllan glucan(SPG) was a extracellular polysaccharides that was from basidiomycete Schizophyllum commune. Schizopyllan was a non-ionic and water soluble compound consisted of a linear chain of 1 6 branched β-(1-3)- glucan loosely attached to the outer layer of cell wall. The rice straw seemed to have the potential to be a substrate media for mushroom cultivation because it consisted a huge lignocellulosic source .Furthermore the large quantity of abundant rice straw was produced during paddy harvesting seasons. Solid state fermentation (SSF) what accommodated the growth of microorganism on moist solid material in the absence of free-flowing water . The objective of this study was to produce the schizophyllan by S.commune fungus from pretreated paddy straw by using solid-sate fermentation process. The Design Expert software (DOE) with application of Central Composite Design (CCD) was used optimization of schizophyllan production . The paddy straw was prepared by grinding into three difference size and other parameters involved were glucose concentration, ammonium nitrate, and amount of Tween 80 was added according to the DOE. The optimization conditions of production of schizophyllan were identified as 10 % glucose, 5 % ammonium nitrate and 0.2 ml Tween 80.