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dc.contributor.authorM. E., Ali
dc.contributor.authorUda, Hashim, Prof. Dr.
dc.contributor.authorShuhaimi, Mustafa
dc.contributor.authorYaakob, Che Man, Dato' Dr.
dc.contributor.authorM. H. M., Yusop
dc.date.accessioned2011-10-04T12:57:46Z
dc.date.available2011-10-04T12:57:46Z
dc.date.issued2011-06-20
dc.identifier.citationp. 384-387en_US
dc.identifier.isbn978-3-6422-1728-9
dc.identifier.issn1680-0737
dc.identifier.urihttp://www.springerlink.com/content/v1g542165vx74353/fulltext.pdf
dc.identifier.urihttp://dspace.unimap.edu.my/123456789/14011
dc.descriptionLink to publisher's homepage at http://www.springerlink.com/en_US
dc.description.abstractA 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene was integrated to a 3-nm diameter citrate- tannate coated gold nanoparticle to fabricate a species specific nanobiosensor. The biosensor was applied to authenticate pork adulteration in autoclaved pork-beef mixtures. The sensor was found to be sensitive enough to detect 0.5 pork in raw and 2.5-h autoclaved mixed samples in a single step without any separation or washing. The hybridization kinetics of the hybrid sensor was studied with synthetic targets from moderate to extreme target concentrations and a sigmoidal relationship was found. The kinetic curve was used to develop a convenient method for quantifying and counting target DNA copy number. The biosensor probe was hybridized with a target DNA that was several-folds shorter than a typical PCR-template. This offered the detection and quantitation of potential targets in highly processed meat products or extensively degraded samples where PCR-based identification technique might not work due to the degradation of comparatively longer DNA. The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences in degraded samples to address a range of biological problems such as food analysis, biodiagnostics, environmental monitoring, genetic screening and forensic investigations.en_US
dc.language.isoenen_US
dc.publisherSpringer-Verlagen_US
dc.relation.ispartofseriesProceedings of the 5th Kuala Lumpur International Conference on Biomedical Engineering (BioMed 2011)en_US
dc.subjectHybrid nanobioprobeen_US
dc.subjectHybridization kineticsen_US
dc.subjectSigmoidal relationshipen_US
dc.subjectSpecies specific nanobiosensoren_US
dc.subjectSynthetic oligo-targetsen_US
dc.titleNanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samplesen_US
dc.typeWorking Paperen_US


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