Document DeliveryDocument Deliveryhttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/382024-03-28T11:55:15Z2024-03-28T11:55:15ZProteins in Mesoporous SilicatesHudson, SarahCooney, JakkiMagner, Edmondhttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/163542011-11-26T07:32:26Z2008-01-01T00:00:00ZProteins in Mesoporous Silicates
Hudson, Sarah; Cooney, Jakki; Magner, Edmond
Mesoporous silicates (MPS) have an ordered pore structure with dimensions comparable to many biological molecules. They have been
extensively explored as supports for proteins and enzymes in biocatalytic
applications. Since their initial discovery, novel syntheses methods have led to precise control over pore size and structure,
particle size, chemical composition, and stability, thus allowing the adsorption of a wide variety of biological macromolecules, such as heme proteins, lipases, antibody fragments, and proteases, into their
structures. This Review discusses the application of ordered, largepore, functionalized mesoporous silicates to immobilize proteins for biocatalysis.
2008-01-01T00:00:00ZApplications of Kinneavy's Theory of Discourse to Technical WritingHarris, Elizabethhttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/163532011-11-26T07:24:54Z1979-02-01T00:00:00ZApplications of Kinneavy's Theory of Discourse to Technical Writing
Harris, Elizabeth
1979-02-01T00:00:00ZCloning, Expression, and Antitumor Activity of Recombinant Protein of CurcinLuo, M. J.Liu, W. X.Yang, X. Y.Xu, Y.Yan, F.Huang, P.Chen, F.http://dspace.unimap.edu.my:80/xmlui/handle/123456789/163522011-11-26T05:33:38Z2007-01-01T00:00:00ZCloning, Expression, and Antitumor Activity of Recombinant Protein of Curcin
Luo, M. J.; Liu, W. X.; Yang, X. Y.; Xu, Y.; Yan, F.; Huang, P.; Chen, F.
Curcin, a protein isolated from the seeds of
Jatropha curcas can be used as a cell-killing agent. To elaborate the purification methods and investigate the antitumor activity of the recombinant protein, the fragment encoding the mature protein of curcin was inserted into E. coli strain M15 and the recombinant strain was induced to express by the optimum inducer (0.5 mM isopropyl-β-D-thiogalactopyranoside). The recombinant protein was expressed in the form of the inclusion body and was purified by Ni-NTA affinity chromatography.
The protein of interest was incubated with the tumor cells at various concentrations for different time. It was shown that the target protein could inhibit the growth of NCL-H446, SGC-7901, and S180 at a very low concentration.
2007-01-01T00:00:00ZDeoxynucleoside Phosphoramidites - a New Class of Key Intermediates for Deoxypolynucleotide SynthesisBeaucage, S.L.Caruthers, M.H.http://dspace.unimap.edu.my:80/xmlui/handle/123456789/163512011-11-26T05:23:11Z1981-01-01T00:00:00ZDeoxynucleoside Phosphoramidites - a New Class of Key Intermediates for Deoxypolynucleotide Synthesis
Beaucage, S.L.; Caruthers, M.H.
1981-01-01T00:00:00Z