Adaptation of compact callus of pomelo (Citrus grandis) to liquid media as potential approach to increase growth

Abstract

Citrus grandis or pomelo is a Malaysia local fruits that found contain high antioxidant compounds such as ascorbic acid and flavonoids. Therefore, the use of young pomelo fruit as explants sources in tissue culture approach for in vitro production of natural antioxidant compounds might sound promising. The study of compounds such as limonene and linalool has been carried out with successful production of those particular compounds in callus culture (Nik Norulaini et. al., 2003). Callus culture of young pomelo fruits was successfully established and the growth was favorable from the explants originated from 5-week old fruits cultured on modified Murashige & Skoog (MS, 1962) media supplemented with 510 mg/l phosphate, 3 mg/l each of 2,4-D and kinetin and 0.2 mg/l ABA (Jenimar, 2001).