Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73353
Title: Optimization of extracellular lipase production by thermotolerant bacteria isolated from a hot spring
Authors: Alli Malar, Harikrishnan
School of Bioprocess Engineering
Issue Date: Jun-2017
Publisher: Universiti Malaysia Perlis (UniMAP)
Abstract: There is high demand for lipase globally where more than 1000 tons of it produced each year. A lipase produced from thermophilic bacteria is revised to have the advantage of thermal stability at 65°C-70°C, which makes them good candidates for lipid modifications which is most effective at high temperature. Thus, main focus of this research is to find the best lipase producing thermotolerant bacteria on its optimum condition of fermentation. The first step on finding lipase producer is to screen the isolated thermotolerant bacterial strains on tributyrin agar plate to check for zone of hydrolysis. Then, followed by screening under submerged fermentation. Next, lipase assay was carried out to find out the best lipase producer among the strains and finally the optimum condition for producing lipase investigated. Among four bacterial strains HS3 showed steady growth and clear zone of hydrolysis. During lipase assay process, HS2 proven to produce higher lipase activity compared to other strains. Therefore, this bacterial strain was chosen of the best lipase producer. During optimization four types of parameter was studied which is carbon source, nitrogen source, oil inducer and temperature. For each parameter there are four variables. Among carbon source Tween 80 proven to has produced highest lipase activity which is 0.09 U/ml. Peptone has shown highest lipase production among nitrogen sources by produced 0.11 U/ml. While from four oil inducers, olive oil strongly agreed to be the highest lipase producer with 0.11U/ml. Within the temperature range of 45-60°C, 55°C is the optimum temperature as it produced lipase activity of 0.09 U/ml. In conclusion, thermotolerant bacteria can produce lipase enzyme under optimum condition with suitable usage of carbon source, nitrogen source, oil inducer and temperature.
Description: Access is limited to UniMAP community.
URI: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73353
Appears in Collections:School of Bioprocess Engineering (FYP)

Files in This Item:
File Description SizeFormat 
Abstract,acknowledgement.pdf158.92 kBAdobe PDFView/Open
Introduction.pdf79.76 kBAdobe PDFView/Open
Literature Review.pdf168.67 kBAdobe PDFView/Open
Methodology.pdf158.41 kBAdobe PDFView/Open
Result and Discussion.pdf494.38 kBAdobe PDFView/Open
Conclusion and Recommendation.pdf70.67 kBAdobe PDFView/Open
Refference and Appendics.pdf110.41 kBAdobe PDFView/Open


Items in UniMAP Library Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.