Antimicrobial activity of aloe vera and Sabah Snake Grass againts Pryricularia Grisea infection and Bacillus bacteria

Abstract

One of the most important cereal crops is rice., particularly in Asia where almost half of the population relies on it as the main food. The ascomycetous fungus Pyricularia oryzae formally known as Pyricularia grisea is causing the most vital and harmful disease of rice which known as rice blast disease. The disease is spread worldwide, but its occurrence and severity differ by year, environmental and location conditions. The symptoms of rice blast can occur on all above ground parts of the plant and is examined at earlier growing stages until the final grain production. A laboratory study is supervised to identify the effect of Aloe Vera and Sabah Snake Grass toward few potential factors which are type of plant sample and concentration of crude extract. Before that, the pathogenic fungi are isolated from the paddy plant and its morphology was identified using microscope. The crude extracts are prepared by differences type of sample which are Aloe Vera and Sabah Snake Grass. The antimicrobial activity identifies by using the disc diffusion technique and minimal inhibition concentration (MIC). To evaluate the MIC, it was demonstrated by using differences concentration of Aloe Vera and Sabah Snake Grass extracts (5 mg/ml to 30 mg/ml). The overall goal of this research is to study the efficacy of Aloe vera and Sabah snake grass leaf extracts against pathogenic fungus Pricularia grisea and Bacillus subtilis.