Computational modeling of Caspase activated DNAase (CAD) regulation towards Apoptosis

Abstract

Caspase activated deoxyribonuclease (CAD) is the protein that transmit nuclear localization signal which will induced DNA fragmentation. Both CAD and its inhibitor ICAD is the key for apoptosis. As CAD is binding on RNA, ICAD-L will bind to its budding chain improving the folding. Once CAD synthesis is done, ICAD-L will still bind with CAD forming DFF which is the CAD: ICAD complex. On the contrary, ICAD-S couldn’t bind with CAD budding chain, therefore do not form DFF. When Caspase-3 is activated downstream in Caspase-3 Cascade, ICAD-L will cleaved and release active CAD to degrade DNA.