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dc.contributor.authorA.L., Ahmad-
dc.contributor.authorC.J.C., Derek-
dc.contributor.authorMohamed Zulkali, Mohamed Daud-
dc.date.accessioned2009-08-04T07:43:29Z-
dc.date.available2009-08-04T07:43:29Z-
dc.date.issued2008-09-22-
dc.identifier.citationSeparation and Purification Technology, vol.62 (3), 2008, pages 702-708.en_US
dc.identifier.issn13835866-
dc.identifier.urihttp://www.sciencedirect.com/science/journal/13835866-
dc.identifier.urihttp://dspace.unimap.edu.my/123456789/6673-
dc.descriptionLink to publisher's homepage at www.elsevier.comen_US
dc.description.abstractThe work attempts to study and optimize aqueous two-phase system (ATPS) made of polyethylene glycol (PEG)/sodium sulfate and PEG/potassium phosphate. Five factors (PEG's molecular weight, PEG's concentration, pH, concentration of sodium chloride and phase-forming salt) affecting the thaumatin partitioning were studied. A two-level fractional factorial was initially carried out. The statistical analysis showed that for both the systems, the phase-forming salt concentration and the concentration of sodium chloride significantly affects the K value for thaumatin partitioning. However, the performance of PEG/sodium sulfate system was generally better than the PEG/potassium phosphate system. Hence a detail study was carried out on the PEG/sodium sulfate system using central composite rotatable design (CCRD) in response surface methodology (RSM). It was observed that high salt concentration increased the K value by sevenfold and the PEG molecular weight was not a significant factor. The optimal condition gave a K value for thaumatin partitioning of 24.15 with a yield of 96.02%.en_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.subjectThaumatinen_US
dc.subjectAqueous two-phase systemen_US
dc.subjectPolyethylene glycol/sodium sulfate systemen_US
dc.subjectTwo-level fractional factorial designen_US
dc.subjectResponse surface methodologyen_US
dc.subjectPolyethylene glycolen_US
dc.subjectPolyethylene glycol -- Biotechnologyen_US
dc.subjectSeparation (Technology)en_US
dc.titleOptimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)en_US
dc.typeArticleen_US
Appears in Collections:School of Bioprocess Engineering (Articles)

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