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http://dspace.unimap.edu.my:80/xmlui/handle/123456789/64924
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DC Field | Value | Language |
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dc.contributor.author | Nurul Atiqah, Ahmad | - |
dc.contributor.author | Ruslinda, A. Rahim | - |
dc.contributor.author | Bohuslav, Rezek | - |
dc.contributor.author | Alexander, Kromka | - |
dc.contributor.author | Nur Syakimah, Ismail | - |
dc.contributor.author | Subash Chandra Bose Gopinath | - |
dc.contributor.author | Tibor, Izak | - |
dc.contributor.author | Vaclav, Prochazka | - |
dc.contributor.author | Fatin Nabilah, Mohd Faudzi | - |
dc.contributor.author | Azrul Syafiq, Zainol Abidin | - |
dc.contributor.author | Nur Nasyifa, Mohd Maidizn | - |
dc.date.accessioned | 2020-06-15T01:35:24Z | - |
dc.date.available | 2020-06-15T01:35:24Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.citation | International Journal of Nanoelectronics and Materials, vol.13(2), 2020, pages 295-306 | en_US |
dc.identifier.issn | 1985-5761 (Printed) | - |
dc.identifier.issn | 1997-4434 (Online) | - |
dc.identifier.uri | http://dspace.unimap.edu.my:80/xmlui/handle/123456789/64924 | - |
dc.description | Link to publisher's homepage at http://ijneam.unimap.edu.my | en_US |
dc.description.abstract | Nanocrystalline diamonds have recently gained great attention to circumvent the current hurdles, with their appealing properties such as high-surface-area to volume ratio, low-background current, wide potential window, biocompatibility, and chemical stability. The nanocrystalline diamonds electrolyte-gated field-effect transistor (NCD-EGFET) can operate directly in solution without involving gate oxides in bringing the hydrogen-tethered moieties and facilitates the p-type surface conductivity. This research investigated on Trans-activator of transcription (Tat) protein; a powerful viral gene activator that plays a pivotal role in the primary stage of the human immunodeficiency virus type 1 (HIV-1) replication. Dose-dependent interactions of HIV-1 Tat on NCD-EGFET-based RNA aptamer sensing surface were monitored and attained the detection down to 10 fM. The linear regression curve with 3σ estimation professed the sensitivity range to be 31.213 mV/log10 [Tat Concentration]M and the limit of detection of 6.18 fM. The selectivity analysis of NCD-EGFET was conducted with different proteins from HIV (Nef and p24) and Bovine Serum Albumin. Furthermore, to practice in the clinical application, HIV-1 Tat was spiked into the human blood serum and it displayed the genuine non-fouling interaction with the aptamer. The attained high-performance signal enhancement with nanocrystalline diamond-biosensing aids to circumvent the issues in the current diagnosis. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Universiti Malaysia Perlis (UniMAP) | en_US |
dc.subject | Aptamer | en_US |
dc.subject | Electrolyte-gated Field Effect Transistor | en_US |
dc.subject | HIV-1 Tat | en_US |
dc.subject | Nanocrystalline Diamonds | en_US |
dc.title | Nanocrystalline diamond electrolyte-gates in field effect transistor for a prolific aptasensing HIV-1 Tat on hydrogen-terminated surface | en_US |
dc.type | Article | en_US |
dc.identifier.url | http://ijneam.unimap.edu.my | - |
dc.contributor.url | ruslinda@unimap.edu.my | en_US |
Appears in Collections: | International Journal of Nanoelectronics and Materials (IJNeaM) |
Files in This Item:
File | Description | Size | Format | |
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Nanocrystalline Diamond Electrolyte-Gates in Field Effect Transistor.pdf | 1.52 MB | Adobe PDF | View/Open |
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