Assessing Antimicrobial Activity of Aloe Vera against Colletrotrichum Capsici and Bacillus Subtilis

Abstract

Nowadays, overuse of chemical fungicide has a side effect which is harmful to human, cause environmental problem and destroys the antagonist microorganisms. Therefore, it is important to develop natural fungicide and bactericide from the plant extract. For this study, chilli anthracnose fungus, Colletotrichum capsici and a pathogenic bacterium Bacillus subtilis were used for assessing the antimicrobial activity. C. capcisi was isolated from the chilli plant and its morphology was identified. Antifungal activity was demonstrated by using different concentrations Aloe vera extract (from 1000 to 5000 ppm). Aloe vera has a lower inhibition of C. capsici at each concentration, which was 1.17 % at 1000 ppm and 7.20 % at 5000 ppm. As for the antibacterial activity, different concentrations of Aloe vera extracts (from 1.25 to 200 mg/ml) were tested on B. subtilis by disc diffusion method. Aloe vera has a higher inhibition of B. subtilis at 200 mg/ml which was 21.33 mm while there is no inhibition zone at 1.25 mg/ml. B. subtilis proved that MIC is 2.5 mg/ml. In conclusion, Aloe vera can be used as the biological fungicide to replace the chemical fungicide. On the other hand, Aloe vera is suitable to be the commercialized as a natural bactericide.