Prolonging glucose sensor's performance in In Vivo environment

Abstract

In this paper, a sol gel based needle type biosensor using 3-glycidoxypropyl dimethylethoxy silane, as silane agent was developed to improve the immobilization efficiency of glucose oxidase (GOD). The glucose sensor was coated with 3C-copolymer as the outmost layer to increase sensor’s biocompatibility. The biosensor’s performance fabricated by this technique was compared with the conventional sensors which use glutaraldehyde as the cross link agent for immobilization of GOD. Both types of sensor were immersed in 20 mM glucose solution for more than 24 hours and the sol gel based sensor exhibited better stability. This indicates that the sol gel immobilization technique applied is able to retain enzyme for longer time of period compared to the conventional method. Furthermore, the study revealed once the sensors were tested in a glucose solution containing 1% bovine serum albumin in a long term stability test, the performance of the sol gel based sensor coated with 3C-copolymer was not affected considerably by protein adsorption, which was a vital character for an in vivo biosensor.