Please use this identifier to cite or link to this item:
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/27694
Title: | Screening and isolation cellulase producing fungi |
Authors: | Siti Hajar, Adnan Saleha Shamsudin |
Keywords: | Lignocellulose Cellulase enzyme Fungi Aspergillus spp |
Issue Date: | May-2012 |
Publisher: | Universiti Malaysia Perlis (UniMAP) |
Abstract: | Three strains fungal were isolated from soil sample Aspergillus spp was the predominant genera in these soil samples.The most potent cellulase producer were selected for stdying their cellulase productivity on rice husk as cheap renewable agriculture waste by solid state fermentation. Aspergillus spp. was grown on selected media for the screening process to see the clearance zone of colony. Result showed that only the fungi type (a) is the best cellulase producer. These fungi were potential cellulose degrader and useful for enzyme production. Fermentation was carried out in flasks containing the different condition of moisture content, ph and inoculums size. These three parameters were studied to find the optimum condition for cellulase production. Optimization of three parameters, initial pH (4.5-5.5), moisture content (65% (w/v)-85% (w/v) and inoculum sizes (2% (w/v)-6% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal cellulase production by fungal type (a). A second order polynomial equation was fitted and the optimum condition was established. The result showed that the optimum condition for cellulase production was predicted at 75% (w/v) initial moisture (5.5) pH and 4% (w/v) inoculum size. The predicted optimal parameter was tested in the laboratory and the cellulase activity 1.55 mg/mL was recorded to be closed to the predicted value is 1.3872 mg/mL. |
Description: | Access is limited to UniMAP community. |
URI: | http://dspace.unimap.edu.my/123456789/27694 |
Appears in Collections: | School of Bioprocess Engineering (FYP) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Abstract, Acknowledgement.pdf | 181.33 kB | Adobe PDF | View/Open | |
Introduction.pdf | 184.18 kB | Adobe PDF | View/Open | |
Literature review.pdf | 262.36 kB | Adobe PDF | View/Open | |
Methodology.pdf | 1.4 MB | Adobe PDF | View/Open | |
Results and discussion.pdf | 995.39 kB | Adobe PDF | View/Open | |
Conclusion.pdf | 106.46 kB | Adobe PDF | View/Open | |
Reference and appendix.pdf | 630.07 kB | Adobe PDF | View/Open |
Items in UniMAP Library Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.