Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/23666
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMd. Eaqub, Ali-
dc.contributor.authorUda, Hashim, Prof. Dr.-
dc.contributor.authorMuhammad, Kashif-
dc.contributor.authorShuhaimi, Mustafa, Assoc. Prof Dr.-
dc.contributor.authorYaakob, Che Man, Prof. Dr.-
dc.date.accessioned2013-02-19T05:49:17Z-
dc.date.available2013-02-19T05:49:17Z-
dc.date.issued2012-06-
dc.identifier.citationGenetic and Molecular Research, 11 (2), 2012, pages 1762-1772.en_US
dc.identifier.issn1676-5680-
dc.identifier.urihttp://www.geneticsmr.com//year2012/vol11-2/pdf/gmr1748.pdf-
dc.identifier.urihttp://dspace.unimap.edu.my/123456789/23666-
dc.descriptionLink to publisher's homepage at http://www.funpecrp.com.br/en_US
dc.description.abstractThe pig (Sus scrofa) mitochondrial genome was targeted to design short (15-30 nucleotides) DNA markers that would be suitable for biosensor-based hybridization detection of target DNA. Short DNA markers are reported to survive harsh conditions in which longer ones are degraded into smaller fragments. The whole swine mitochondrial-genome was in silico digested with AluI restriction enzyme. Among 66 AluI fragments, five were selected as potential markers because of their convenient lengths, high degree of interspecies polymorphism and intraspecies conservatism. These were confirmed by NCBI blast analysis and ClustalW alignment analysis with 11 different meat-providing animal and fish species. Finally, we integrated a tetramethyl rhodamine-labeled 18-nucleotide AluI fragment into a 3-nm diameter citrate-tannate coated gold nanoparticle to develop a swine-specific hybrid nanobioprobe for the determination of pork adulteration in 2.5-h autoclaved pork-beef binary mixtures. This hybrid probe detected as low as 1% pork in deliberately contaminated autoclaved pork-beef binary mixtures and no cross-species detection was recorded, demonstrating the feasibility of this type of probe for biosensor-based detection of pork adulteration of halal and kosher foods.en_US
dc.language.isoenen_US
dc.publisherFundacao de Pesquisas Cientificas de Ribeirao Preto (FUNPEC-RO)en_US
dc.subjectAmplicon lengthen_US
dc.subjectIn silico digestionen_US
dc.subjectIntra- and interspecies polymorphismsen_US
dc.subjectMitochondrial genomeen_US
dc.subjectProtein coding genesen_US
dc.subjectRestriction enzymeen_US
dc.titleDevelopment of swine-specific DNA markers for biosensor-based halal authenticationen_US
dc.typeArticleen_US
dc.contributor.urleaqubali@gmail.comen_US
dc.contributor.urluda@unimap.edu.myen_US
dc.contributor.urlkashif_bme@yahoo.comen_US
dc.contributor.urlshuhaimi@biotech.upm.edu.myen_US
dc.contributor.urlycm@putra.upm.edu.myen_US
Appears in Collections:Uda Hashim, Prof. Ts. Dr.
Institute of Nano Electronic Engineering (INEE) (Articles)

Files in This Item:
File Description SizeFormat 
Development of swine-specific DNA markers for biosensor-based halal authentication.pdf1.03 MBAdobe PDFView/Open


Items in UniMAP Library Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.