Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/18753
Full metadata record
DC FieldValueLanguage
dc.contributor.authorFhataheya, Buang-
dc.contributor.authorMukhzeer, Mohamad Shahimin, Dr.-
dc.date.accessioned2012-04-10T12:36:09Z-
dc.date.available2012-04-10T12:36:09Z-
dc.date.issued2010-11-30-
dc.identifier.citationp. 176-179en_US
dc.identifier.isbn978-1-4244-7600-8-
dc.identifier.urihttp://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=5742223-
dc.identifier.urihttp://dspace.unimap.edu.my/123456789/18753-
dc.descriptionLink to publisher's homepage at http://www.ieee.org/en_US
dc.description.abstractMammalian adipose tissue derived stem cells have a tremendous potential in regenerative medicine for tissue engineering and somatic nuclear transfer (SNT). The isolation methods of human and bovine adipose tissue derived stem cells are compared in this paper to determine the feasibility and optimum method of isolation. The optimum isolation method will reduce the processing time, efforts and money as isolation is the first crucial and important step in stem cells research. Human abdominal subcutaneous adipose tissue and bovine abdominal subcutaneous adipose tissue are digested in three collagenase type 1 concentration 0.075%, 0.3% and 0.6% agitated at 1 hour and 2 hours under 37°C in 5% CO2 incubator. The cultures are then morphologically characterised. Human adipose tissue stem cells are found to be best isolated using abdominal subcutaneous depot, using 0.075% collagenase type 1 agitated at 1 hours under 37C in CO2 incubator. While bovine adipose tissue derived stem cells are best isolated using abdominal subcutaneous depot, using 0.6% collagenase type 1 agitated at 2 hours under 37°C in CO2 incubator.en_US
dc.language.isoenen_US
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en_US
dc.relation.ispartofseriesProceedings of 2010 IEEE EMBS Conference on Biomedical Engineering and Sciences (IECBES) 2010en_US
dc.subjectAbdominal subcutaneous adipose tissueen_US
dc.subjectBovineen_US
dc.subjectHumanen_US
dc.subjectStem cell isolationen_US
dc.titleIsolation methods of human and bovine adipose tissue derived stem cellsen_US
dc.typeWorking Paperen_US
dc.contributor.urlmukhzeer@unimap.edu.myen_US
dc.contributor.urlfhataheya_buang@yahoo.comen_US
Appears in Collections:Conference Papers
Mukhzeer Mohamad Shahimin, Dr.

Files in This Item:
File Description SizeFormat 
Isolation methods of human and bovine adipose.pdf7.96 kBAdobe PDFView/Open


Items in UniMAP Library Digital Repository are protected by copyright, with all rights reserved, unless otherwise indicated.