• Login
    View Item 
    •   DSpace Home
    • Researchers
    • Farrah Aini Dahalan, Dr.
    • View Item
    •   DSpace Home
    • Researchers
    • Farrah Aini Dahalan, Dr.
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil

    Thumbnail
    View/Open
    Growth kinetics of a diesel-degrading bacterial strain from petroleum-contaminated soil.pdf (34.96Kb)
    Growth kinetics of a diesel-degrading.pdf (622.3Kb)
    Date
    2014-03
    Author
    Farrah Aini, Dahalan
    Ibrahim, Yunus
    Wan Lutfi, Wan Johari
    Mohd Yunus, Shukor
    Mohd Izuan Effendi, Halmi
    Nor Aripin, Shamaan
    Mohd Arif, Syed
    Metadata
    Show full item record
    Abstract
    A diesel-degrading bacterium was isolated from a diesel-contaminated site in Selangor, Malaysia. The isolate was tentatively identified as Acinetobacter sp. strain DRY12 based on partial 16S rDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Optimum growth occurred from 3 to 5% diesel and the strain was able to tolerate as high as 8% diesel. The optimal pH that supported growth of the bacterium was between pH 7.5 to 8.0. The isolate exhibited optimal growth in between 30 and 35° C. The best nitrogen source was potassium nitrate (between 0.6 and 0.9% (w/v)) followed by ammonium chloride, sodium nitrite and ammonium sulphate in descending order. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 10 days of incubation. The best growth kinetic model to fit experimental data was the Haldane model of substrate inhibiting growth with a correlation coefficient value of 0.97. The maximum growth rate- μmax was 0.039 hr -1 while the saturation constant or half velocity constant Ks and inhibition constant Ki, were 0.387% and 4.46%, respectively. MATH assays showed that 75% of the bacterium was found in the hexadecane phase indicating that the bacterium was hydrophobic. The characteristics of this bacterium make it useful for bioremediation works in the Tropics.
    URI
    http://www.jeb.co.in/journal_issues/201403_mar14/paper_16.pdf
    http://dspace.unimap.edu.my:80/xmlui/handle/123456789/41168
    Collections
    • Farrah Aini Dahalan, Dr. [15]

    Atmire NV

    Perpustakaan Tuanku Syed Faizuddin Putra (PTSFP) | Send Feedback
     

     

    Browse

    All of UniMAP Library Digital RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Atmire NV

    Perpustakaan Tuanku Syed Faizuddin Putra (PTSFP) | Send Feedback