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dc.contributor.authorNursabrina, Mohd Hayat
dc.contributor.authorNor Aripin, Shamaan
dc.contributor.authorMohd Yunus, Shukor
dc.contributor.authorMohd Khalizan, Sabullah
dc.contributor.authorMohd. Arif, Syed
dc.contributor.authorFarrah Aini, Dahalan
dc.contributor.authorKhalilalh, Abdul Khalil
dc.contributor.authorSiti Aqlima, Ahmad
dc.date.accessioned2016-03-21T08:40:18Z
dc.date.available2016-03-21T08:40:18Z
dc.date.issued2015-04-01
dc.identifier.citationJournal of Chemical and Pharmaceutical Sciences, vol. 8 (2), 2015, pages 376-381en_US
dc.identifier.issn0974-2115
dc.identifier.urihttp://jchps.com/past.html#v8is2
dc.identifier.urihttp://dspace.unimap.edu.my:80/xmlui/handle/123456789/41166
dc.descriptionLink to publisher's homepage at http://jchps.com/en_US
dc.description.abstractHeavy metals are sometimes needed for nutrient uptake but only in low concentrations and the nervous system is the most susceptible to be affected. Cholinesterase (ChE) inhibition has been used extensively as a biomarker for heavy metals detection. In this study, the potential of ChE from Lates calcarifer brain as an alternative biosensor for heavy metals is evaluated. There are many Malaysian rivers such as Sungai Juru and Sungai Merbok that are greatly polluted by industrial effluents. Chronic exposure to heavy metals may cause nervous system disorders. Thus, a fast and simple biomonitoring technique will help in alerting government agencies and the public to such a threat. In this study, ChE from the brain of L. calcarifer (Asian seabass) was purified through ammonium sulphate precipitation and ion exchange chromatography. Enzyme recovery was 5.31% with a specific activity of 5.472 U mg-1. The Michaelis-Menten constant (Km) value was 0.3075 mM and a Vmax of 0.0304 μmol min-1 mg-1. ChE from L. calcarifer brain showed higher affinity to acetylthiocholine iodide (ATC) compared to butyrylcholinesterase (BTC) and propionylcholinesterase iodide (PTC). Maximum activity of ChE was detected when the enzyme was assayed at the temperature of 20-30°C and incubated in Tris-HCl buffer pH 8.0. Silver (Ag), arsenic (As), cadmium (Cd), chromium (Cr) and mercury (Hg) showed more than 50% inhibition amongst the 10 types of heavy metals that have been tested for inhibition study. These results can be used to further develop an alternative way to detect heavy metals that are low cost and give faster results compared to existing biosensor kits.en_US
dc.language.isoenen_US
dc.publisherSPB Pharma Societyen_US
dc.subjectBiomarkeren_US
dc.subjectBiomonitoringen_US
dc.subjectCholinesteraseen_US
dc.subjectHeavy metalsen_US
dc.titleCholinesterase-based biosensor using Lates calcarifer (Asian Seabass) brain for detection of heavy metalsen_US
dc.typeArticleen_US
dc.contributor.urlfarrahaini@unimap.edu.myen_US
dc.contributor.urlaqlima@upam.edu.myen_US


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