Ku Syahidah Ku Ismail, Dr.
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/39185
2024-03-29T16:00:16ZCo-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/32778
Co-expression of TAL1 and ADH1 in recombinant xylose-fermenting Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysates in the presence of furfural
Hasunuma, Tomohisa; Ku Syahidah, Ku Ismail; Nambu, Yumiko; Kondo, Akihiko
Lignocellulosic biomass dedicated to bioethanol production usually contains pentoses and inhibitory compounds such as furfural that are not well tolerated by Saccharomyces cerevisiae. Thus, S. cerevisiae strains with the capability of utilizing both glucose and xylose in the presence of inhibitors such as furfural are very important in industrial ethanol production. Under the synergistic conditions of transaldolase (TAL) and alcohol dehydrogenase (ADH) overexpression, S. cerevisiae MT8-1X/TAL–ADH was able to produce 1.3-fold and 2.3-fold more ethanol in the presence of 70 mM furfural than a TAL-expressing strain and a control strain, respectively. We also tested the strains' ability by mimicking industrial ethanol production from hemicellulosic hydrolysate containing fermentation inhibitors, and ethanol production was further improved by 16% when using MT8-1X/TAL–ADH compared to the control strain. Transcript analysis further revealed that besides the pentose phosphate pathway genes TKL1 and TAL1, ADH7 was also upregulated in response to furfural stress, which resulted in higher ethanol production compared to the TAL-expressing strain. The improved capability of our modified strain was based on its capacity to more quickly reduce furfural in situ resulting in higher ethanol production. The co-expression of TAL/ADH genes is one crucial strategy to fully utilize undetoxified lignocellulosic hydrolysate, leading to cost-competitive ethanol production.
Link to publisher's homepage at http://www.elsevier.com
2014-02-01T00:00:00ZEffect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/21210
Effect of nutrient modification on growth and flavonone production from callus culture of Citrus grandis
Azlinda, Abdul Ghani; Zarina, Zakaria, Dr.; Ku Syahidah, Ku Ismail; Nor Alina, Nazri
This paper discus on the production of flavonones, one of the flavonoids compounds that correlated with antioxidants activities in callus culture of pomelo. This is an alternative strategy of producing natural antioxidants without sacrificing part of plants. Flavonones was detected as naringin and hesperidin using High Performance Liquid Chromatography (HPLC). There are three types of media were used to investigate their capability to produce the highest callus biomass. It was found that growth of callus was preferable on medium supplied with 1925 mg/l of nitrate and 60 g/l of sucrose. For both combinations, the result showed that it can give the best hesperidin accumulation (22.861 and 69.923 mg/l). The maximum productivity of hesperidin can be accumulated by seven days of incubation period. Naringin however was maximize with supplementation of 1925 mg/l nitrate (18.676 mg/l) and 90 g/l (33.876 mg/l) sucrose within the same duration time of incubation period.
International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Kampus Pauh Putra, Arau, Perlis, Malaysia.
2010-10-16T00:00:00ZBioethanol from cassava: Characterization of starch during thermo-enzymatic hydrolysis
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/15107
Bioethanol from cassava: Characterization of starch during thermo-enzymatic hydrolysis
Ku Syahidah, Ku Ismail; Noorulnajwa Diyana, Yaacob; Khairul Farihan, Kasim; Khairul Akhbar, Ahmad Zabidi; Mohamed Zulkali, Mohamed Daud, Prof. Dr.
One of the greatest challenges for the 21" century society is to meet the growing demand for energy for transportation in a sustainable way. High prices of petroleum in the market are in critical state and alternative for fuel is needed. Therefore, cassava is chosen as the raw material for ethanol production via enzymatic hydrolysis and fermentation. This paper discusses the fundamental characterization of starch degradation during thermo-enzymatic hydrolysis. In this project, non edible cassava is used as the raw material for starch, which undergoes enzymatic hydrolysis to produce glucose. By using various analytical equipments, its characteristic during hydrolysis was determined. It was found thai the root starches has a polyhedric shape by visualizing under SEM. The surface of this starch was slightly rough which contains pinholes and protrusions as confirmed by using AFM. Under XRD, the pattern shows that the cassava was classified as B-type starch and their gelatinization temperature was high which is 89.4°C.
Organized by Universiti Teknologi MARA, 12th - 13th December 2008 at Penang, Malaysia.
2008-12-12T00:00:00ZCiTRUsX pomelo tissue culture for natural antioxidant enrichment in food and health products
http://dspace.unimap.edu.my:80/xmlui/handle/123456789/9559
CiTRUsX pomelo tissue culture for natural antioxidant enrichment in food and health products
Zarina, Zakaria, Dr.; Ku Syahidah, Ku Ismail; Khairul Farihan, Kassim; Alina Rahayu, Mohamed; Azlinda, Ali; Nor Alina, Nazri
Production of natural antioxidant via tissue culture technique.
Organized by Korea Invention Promotion Association (KIPA), 3rd - 7th December 2009 at Pacific Hall, COEX, Korea.
2009-12-03T00:00:00Z