iRepository at Perpustakaan UniMAP >
Journal Articles >
Journal of Engineering Research and Education (JERE) >

Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/7450

Title: Improvement of Glucose Oxidase Immobilization using Sol-Gel technique
Authors: Nashrul Fazli, Mohd Nasir
Yang, Y.
???metadata.dc.contributor.url???: nashrul@unimap.edu.my
Keywords: Biosensor;Glucose sensor;Immobilization technique;Sol gel technique;Hiocompatibility
Issue Date: 2007
Publisher: Universiti Malaysia Perlis
Citation: Journal of Engineering Research and Education, vol. 4, 2007, pages 62-73.
Abstract: A sol gel based needle type biosensor using 3-glycidoxypropyl dimethylethoxy silane, as silane agent was developed to improve the immobilisation efficiency of glucose oxidase (GOD). The glucose sensor was coated with 3C-copolymer as outmost layer to increase sensor's bioconipatibility. The biosensor's performance fabricated by this technique was compared with the conventional sensors which uses glntaraldehyde as the cross link agent for immobilisation of GOD. Reliable results were obtained from various characterisation tests, including sensor response to glucose, the sensitivity and stability tests. In the stability test, both types of the sensors were immersed in 20 mM glucose solution for more than 24 hours and the sol gel based sensor showed a better stability. This indicates that the sol gel immobilisation technique applied is able to retain ensyme for longer time of period compared to the conventional method. Furthermore, the studies revealed when the sensors were tested in a glucose solution containing 1 % bovine serum albumin in a long term stability test, the performance of the sol gel based sensor coated with 3C-copolymer was not affected considerably by protein adsorption, which is a vital character for an in vivo biosensor.
Description: Link to publisher's homepage at http://jere.unimap.edu.my
URI: http://jere.unimap.edu.my
ISSN: 1823-2981
Appears in Collections:Nashrul Fazli Mohd Nasir, Dr.
Journal of Engineering Research and Education (JERE)

Files in This Item:

File Description SizeFormat
Improvement of Glucose Oxidase.pdfAccess is limited to UniMAP community.7.04 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! Perpustakaan Tuanku syed Faizuddin Putra, Kampus Pauh Putra, Universiti Malaysia Perlis, 02600, Arau Perlis
TEL: +604-9885420 | FAX: +604-9885405 | EMAIL: rujukan@unimap.edu.my Feedback