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http://hdl.handle.net/123456789/21499
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| Title: | NanoDNA chip for the detection of porcine derivatives in halal products |
| Authors: | Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Md. Fazlul, Bari, Dr. |
| E-mail: | eaqubali@gmail.com |
| Keywords: | NanoDNA chip
Label free electrical detection
Nanogap electrodes
Conserved sequences
Spacer process lithography
AluI sites
AluI digestion
Polymorphism
Blast
Alignment analysis |
| Issue Date: | 16-Oct-2010 |
| Publisher: | Universiti Malaysia Perlis (UniMAP) |
| Department: | Centre for Graduate Studies |
| Series/Report no.: | Proceedings of the International Postgraduate Conference on Engineering (IPCE 2010) |
| Abstract: | Pig (Sus scrofa) mitochondrial genome
was analyzed for AluI sites by NEB cutter version 2
and REBASE softwares. Out of 66 AluI fragments,
five were selected as probes for the construction of
porcine mitochondrial DNA chip because of their
convenient sizes, highly conserved unique specificity
for porcine mitochondrial genome and polymorphism
with other species. These were confirmed by NCBI
blast analysis and ClustalW alignment analysis.
These AluI cut fragments are proposed to be
immobilized on nanogap electrodes to be fabricated
by spacer process lithography to construct NanoDNA
chip. Application of AluI digested denatured ssDNA
on the chip will immediately hybridized with the
immobilized probes to form dsDNA to bring
electronic and electrical changes in the dielectric
properties of the molecule. The electronic and
electrical signals will be read by a dielectric analyzer
to detect the presence of porcine DNA with any
additional labeling steps. |
| Description: | International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia. |
| URI: | http://hdl.handle.net/123456789/21499 |
| ISBN: | 978-967-5760-03-7 |
| Appears in Collections: | Conference Papers
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