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Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/21499

Title: NanoDNA chip for the detection of porcine derivatives in halal products
Authors: Md. Eaqub, Ali
Uda, Hashim, Prof. Dr.
Md. Fazlul, Bari, Dr.
???metadata.dc.contributor.url???: eaqubali@gmail.com
Keywords: NanoDNA chip;Label free electrical detection;Nanogap electrodes;Conserved sequences;Spacer process lithography;AluI sites;AluI digestion;Polymorphism;Blast;Alignment analysis
Issue Date: 16-Oct-2010
Publisher: Universiti Malaysia Perlis (UniMAP)
???metadata.dc.publisher.department???: Centre for Graduate Studies
Series/Report no.: Proceedings of the International Postgraduate Conference on Engineering (IPCE 2010)
Abstract: Pig (Sus scrofa) mitochondrial genome was analyzed for AluI sites by NEB cutter version 2 and REBASE softwares. Out of 66 AluI fragments, five were selected as probes for the construction of porcine mitochondrial DNA chip because of their convenient sizes, highly conserved unique specificity for porcine mitochondrial genome and polymorphism with other species. These were confirmed by NCBI blast analysis and ClustalW alignment analysis. These AluI cut fragments are proposed to be immobilized on nanogap electrodes to be fabricated by spacer process lithography to construct NanoDNA chip. Application of AluI digested denatured ssDNA on the chip will immediately hybridized with the immobilized probes to form dsDNA to bring electronic and electrical changes in the dielectric properties of the molecule. The electronic and electrical signals will be read by a dielectric analyzer to detect the presence of porcine DNA with any additional labeling steps.
Description: International Postgraduate Conference On Engineering (IPCE 2010), 16th - 17th October 2010 organized by Centre for Graduate Studies, Universiti Malaysia Perlis (UniMAP) at School of Mechatronic Engineering, Pauh Putra Campus, Perlis, Malaysia.
URI: http://hdl.handle.net/123456789/21499
ISBN: 978-967-5760-03-7
Appears in Collections:Md. Fazlul Bari, Assoc. Prof. Dr.
Conference Papers
Uda Hashim, Prof. Dr.

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