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Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/dspace/handle/123456789/14011

Title: Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples
Authors: M. E., Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa
Yaakob, Che Man, Dato' Dr.
M. H. M., Yusop
Keywords: Hybrid nanobioprobe;Hybridization kinetics;Sigmoidal relationship;Species specific nanobiosensor;Synthetic oligo-targets
Issue Date: 20-Jun-2011
Publisher: Springer-Verlag
Citation: p. 384-387
Series/Report no.: Proceedings of the 5th Kuala Lumpur International Conference on Biomedical Engineering (BioMed 2011)
Abstract: A 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene was integrated to a 3-nm diameter citrate- tannate coated gold nanoparticle to fabricate a species specific nanobiosensor. The biosensor was applied to authenticate pork adulteration in autoclaved pork-beef mixtures. The sensor was found to be sensitive enough to detect 0.5 pork in raw and 2.5-h autoclaved mixed samples in a single step without any separation or washing. The hybridization kinetics of the hybrid sensor was studied with synthetic targets from moderate to extreme target concentrations and a sigmoidal relationship was found. The kinetic curve was used to develop a convenient method for quantifying and counting target DNA copy number. The biosensor probe was hybridized with a target DNA that was several-folds shorter than a typical PCR-template. This offered the detection and quantitation of potential targets in highly processed meat products or extensively degraded samples where PCR-based identification technique might not work due to the degradation of comparatively longer DNA. The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences in degraded samples to address a range of biological problems such as food analysis, biodiagnostics, environmental monitoring, genetic screening and forensic investigations.
Description: Link to publisher's homepage at http://www.springerlink.com/
URI: http://www.springerlink.com/content/v1g542165vx74353/fulltext.pdf
http://hdl.handle.net/123456789/14011
ISBN: 978-3-6422-1728-9
ISSN: 1680-0737
Appears in Collections:Conference Papers
Uda Hashim, Prof. Dr.

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