iRepository at Perpustakaan UniMAP >
The Library >
Conference Papers >

Please use this identifier to cite or link to this item: http://dspace.unimap.edu.my:80/xmlui/handle/123456789/14011

Title: Nanobiosensor for the detection and quantification of specific DNA sequences in degraded biological samples
Authors: M. E., Ali
Uda, Hashim, Prof. Dr.
Shuhaimi, Mustafa
Yaakob, Che Man, Dato' Dr.
M. H. M., Yusop
Keywords: Hybrid nanobioprobe;Hybridization kinetics;Sigmoidal relationship;Species specific nanobiosensor;Synthetic oligo-targets
Issue Date: 20-Jun-2011
Publisher: Springer-Verlag
Citation: p. 384-387
Series/Report no.: Proceedings of the 5th Kuala Lumpur International Conference on Biomedical Engineering (BioMed 2011)
Abstract: A 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene was integrated to a 3-nm diameter citrate- tannate coated gold nanoparticle to fabricate a species specific nanobiosensor. The biosensor was applied to authenticate pork adulteration in autoclaved pork-beef mixtures. The sensor was found to be sensitive enough to detect 0.5 pork in raw and 2.5-h autoclaved mixed samples in a single step without any separation or washing. The hybridization kinetics of the hybrid sensor was studied with synthetic targets from moderate to extreme target concentrations and a sigmoidal relationship was found. The kinetic curve was used to develop a convenient method for quantifying and counting target DNA copy number. The biosensor probe was hybridized with a target DNA that was several-folds shorter than a typical PCR-template. This offered the detection and quantitation of potential targets in highly processed meat products or extensively degraded samples where PCR-based identification technique might not work due to the degradation of comparatively longer DNA. The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences in degraded samples to address a range of biological problems such as food analysis, biodiagnostics, environmental monitoring, genetic screening and forensic investigations.
Description: Link to publisher's homepage at http://www.springerlink.com/
URI: http://www.springerlink.com/content/v1g542165vx74353/fulltext.pdf
ISBN: 978-3-6422-1728-9
ISSN: 1680-0737
Appears in Collections:Conference Papers
Uda Hashim, Prof. Dr.

Files in This Item:

File Description SizeFormat
nanibiosensor.pdf8.3 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! Perpustakaan Tuanku syed Faizuddin Putra, Kampus Pauh Putra, Universiti Malaysia Perlis, 02600, Arau Perlis
TEL: +604-9885420 | FAX: +604-9885405 | EMAIL: rujukan@unimap.edu.my Feedback