|
iRepository at Perpustakaan UniMAP >
FINAL YEAR PROJECTS >
School of Bioprocess Engineering (FYP) >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/13364
|
| Title: | Development of disposable Xanthine Oxidase biosensor for the determination of antioxidant properties of herbal teas |
| Authors: | Mohamad Syafiq, Sa’odi |
| Advisors: | Dr. AKM Shafiqul Islam (Advisor) |
| Keywords: | Enzymatic biosensor
Xanthine Oxidase (XO)
Antioxidant
Herbal tea
Medicinal plants
Biosensor |
| Issue Date: | May-2011 |
| Publisher: | Universiti Malaysia Perlis |
| Department: | School of Bioprocess Engineering |
| Abstract: | An enzymatic biosensor has been developed using Xanthine Oxidase (XO enzyme as
sensing material for the determination of antioxidant properties of selected herbal teas. The
enzyme was immobilized in a Kappa-Carrageenan membrane on a carbon paste electrode.
The variables concerning the biosensor preparation such as types of tea, amount of Kappa-
Carrageenan membrane, concentration of tea samples, addition of Xanthine solution and tea
samples, and also addition of supporting electrolytes were studied. The antioxidant
properties of the herbal teas were demonstrated by comparing percentage of inhibition of
selected tea samples using the standard 1, 1-diphenyl-2-picrylhydrazyl DPPH free radical
scavenging potential and with the cyclic voltammogtram using the Dropsens biosensor.
Results show that cyclic voltammetry analysis using the Xanthine Oxidase biosensor
produced significant result in comparison with the free radical scavenging activity using
DPPH. Even though the results using biosensor is less accurate compared to standard
method, but it was fairly acceptable. |
| Description: | Access is limited to UniMAP community. |
| URI: | http://hdl.handle.net/123456789/13364 |
| Appears in Collections: | School of Bioprocess Engineering (FYP)
|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
|
